Sam Lord's Publications

grid  cell superres


23. High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
Charles-Orszag, A.; Lord, S. J.; Mullins, R. D. Front. Microbiol. 2021, 12, 707124.

22. SuperPlots: Communicating reproducibility and variability in cell biology
Lord, S. J.; Velle, K. B.; Mullins, R. D.; Fritz-Laylin, L. K. J. Cell Biol. 2020, 219(6), e202001064.

21. Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
Yang, B. et al. Nature Methods 2019, 16, 501–504.

20. Editorial: Concise Language Promotes Clear Thinking about Cell Shape and Locomotion
Fritz-Laylin, L. K.; Lord, S. J.; Kakley, M.; Mullins, R. D. Bioessays 201840(7):e1700225.

19. Actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes
Fritz-Laylin, L. K. et al. eLife 20176, e26990.

18. Editorial: Our evolving view of cell motility
Fritz-Laylin, L. K.; Lord, S. J.; Mullins, R. D. Cell Cycle 201716(19), 1735-1736.

17. WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility spotlighted in JCB; top 10 articles in JCB in 2017selected for Cytoskeletal Dynamics issue
Fritz-Laylin, L. K.; Lord, S. J.; Mullins, R. D. J. Cell Biol. 2017, 216(6), 1673-1688.

16. Spatial Organization of EphA2 at the Cell-Cell Interface Modulates Trans-Endocytosis of EphrinA1
Greene, A. C.;* Lord, S. J.;* Tian, A.; Rhodes, C.; Kai, H.; Groves, J. T. Biophys. J. 2014, 106(10), 2196-2205. *equal contribution

15. Azido Push-Pull Fluorogens Photoactivate to Produce Bright Fluorescent Labels
Lord, S. J.; Lee, H. D.; Samuel, R.; Weber, R.; Liu, N.; Conley, N. R.; Thompson, M. A.; Twieg, R. J.; Moerner, W. E. J. Phys. Chem. B 2010, 114(45), 14157-14167.

14. Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores highlighted in Nature; evaluated by F1000
Lee, H. D.; Lord, S. J.; Iwanaga, S.; Zhan, K.; Xie, H.; Williams, J. C.; Wang, H.; Bowman, G. R.; Goley, E. D.; Shapiro, L.; Twieg, R. J.; Rao, J.; Moerner W. E. J. Am. Chem. Soc. 2010, 132(43), 15099-15101.

13. Review: Molecules and Methods for Super-Resolution Imaging
Thompson, M. A.; Biteen, J. S.; Lord, S. J.; Conley, N. R.; Moerner, W. E. Methods Enzym. 2010, 475, 27-59.

12. Review: Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells
Lord, S. J.; Lee, H. D.; Moerner, W. E. Anal. Chem. 2010, 82(6), 2192-2203.

11. Three-Dimensional Single-Molecule Fluorescence Imaging Beyond the Diffraction Limit Using a Double-Helix Point Spread Function
Pavani, S. R. P. et al. Proc. Natl. Acad. Sci. U.S.A. 2009, 106(9), 2995-2999.

10. Bright, Red Single-Molecule Emitters: Synthesis and Properties of Environmentally Sensitive Dicyanomethylenedihydrofuran (DCDHF) Fluorophores with Bisaromatic Conjugation
Lu, Z.; Liu, N.; Lord, S. J.; Willets, K. A.; Moerner, W. E.; Twieg, R. J. Chem. Mater. 2009, 21(5), 797-810.

9. Review: DCDHF Fluorophores for Single-Molecule Imaging in Cells
Lord, S. J.; Conley, N. R.; Lee, H. D.; Nishimura, S. Y.; Pomerantz, A. K.; Willets, K. A.; Lu, Z.; Wang, H.; Liu, N.; Samuel, R.; Weber, R.; Semyonov, A.; He, M.; Twieg, R. J.; Moerner, W. E. ChemPhysChem 2009, 10(1), 55-65.

8. A Photoactivatable Push-Pull Fluorophore for Single-Molecule Imaging in Live Cells highlighted in C&E News
Lord, S. J.; Conley, N. R.; Lee, H. D.; Samuel, R.; Liu, N.; Twieg, R. J.; Moerner, W. E. J. Am. Chem. Soc. 2008, 130(29), 9204-9205.

7. Chapter: Nanophotonics and Single Molecules (HTML, PDF)
Moerner, et al. Chapter in Single Molecules and Nanotechnology; R. Rigler and H. Vogel, Eds.; Springer Series in Biophysics, Vol. 12; Springer-Verlag: Berlin, 2008; pp 1-23.

6. Photophysical Properties of Acene DCDHF Fluorophores: Long-Wavelength Single-Molecule Emitters Designed for Cellular Imaging
Lord, S. J.; Lu, Z.; Wang, H.; Willets, K. A.; Schuck, P. J; Lee, H. D.; Nishimura, S. Y.; Twieg, R. J.; Moerner, W. E. J. Phys. Chem. A 2007, 111(37), 8934-8941.

5. Modifications of DCDHF Single Molecule Fluorophores to Impart Water Solubility
Wang, H.; Lu, Z.; Lord, S. J.; Moerner, W. E.; Twieg, R. J. Tetrahedron Lett. 2007, 48(19), 3471-3474.

4. The influence of tetrahydroquinoline rings in dicyanomethylenedihydrofuran (DCDHF) single-molecule fluorophores (HTML, PDF)
Wang, H; Lu, Z.; Lord, S. J.; Willets, K. A.; Bertke, J. A.; Bunge, S. D.; Moerner, W. E.; Twieg, R. J. Tetrahedron 2007, 63(1), 103-114.

3. A Long-Wavelength Analogue of PRODAN: Synthesis and Properties of Anthradan, a Fluorophore with a 2,6-Donor-Acceptor Anthracene Structure
Lu, Z.; Lord, S. J.; Wang, H.; Moerner, W. E.; Twieg, R. J. J. Org. Chem. 2006, 71(26), 9651-9657.

2. Diffusion of Lipid-like Single-Molecule Fluorophores in the Cell Membrane
Nishimura, S. Y.; Lord, S. J.; Klein, L. O.; Willets, K. A.; Lu, Z.; He, M.; Twieg, R. J.; Moerner, W. E. J. Phys. Chem. B 2006, 110(15), 8151-8157.

1. Tadpole conformation of gradient polymer brushes (HTML, PDF)
Lord, S. J.; Sheiko, S. S.; LaRue, I.; Lee, H. I.; Matyjaszewski, K. Macromolecules 2004, 37(11), 4235-4240.


Patents:

2. Fluorogenic Compounds Converted to Fluorophores by Photochemical or Chemical Means and Their Use in Biological Systems (HTML, PDF)
Twieg, R. J.; Moerner, W. E.; Lord, S. J.; Liu, N.; Samuel, R. US Patent 8,772,048. Filed March 1, 2012; issued July 8, 2014.

1. Fluorogenic Compounds Converted to Fluorophores by Photochemical or Chemical Means and Their Use in Biological Systems (HTML, PDF)
Twieg, R. J.; Moerner, W. E.; Lord, S. J.; Liu, N.; Samuel, R. US Patent 8,153,446. Filed May 13, 2009; issued April 10, 2012.


Dissertation: Fluorophores for Single-Molecule Imaging in Living Cells: Characterizing and Optimizing DCDHF Photophysics (HTML, PDF)
Lord, S. J. Ph.D. Dissertation, Stanford University, March 2010.


Preprints:

7. Peer Replication
Lord et al. Zenodo 2023. DOI: 10.5281/zenodo.10067391

6. Sulfolobus acidocaldarius adhesion pili power twitching motility in the absence of a dedicated retraction ATPase
Charles-Orszag et al. bioRxiv 2023. DOI: 10.1101/2023.08.04.552066

5. Quantitative proteomic analysis reveals apoE4-dependent phosphorylation of the actin-regulating protein VASP
Cakir et al. bioRxiv 2022. DOI: 10.1101/2022.06.06.495052

4. High-temperature live-cell imaging of cytokinesis, cell motility and cell-cell adhesion in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius
Charles-Orszag  et al. bioRxiv 2020. DOI: 10.1101/2020.02.16.951772

3. If your P value looks too good to be true, it probably is: Communicating reproducibility and variability in cell biology
Lord et al. arXiv 2019, 1911.03509.

2. Three-dimensional actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes
Fritz-Laylin et al. bioRxiv 2017. DOI: 10.1101/120444

1. Chytrid fungi construct actin-rich pseudopods, implicating actin regulators WASP and SCAR in an ancient mode of cell motility
Fritz-Laylin, et al. bioRxiv 2016. DOI: 10.1101/051821